Scientists Perfect Creation of Artificial Spider Silk

Biomaterials

Producing artificial spider silk has long been a dream of many scientists. Now, a team of researchers claims to have developed a method that works. They report that they can produce kilometer long threads that resemble real spider silk.

Being able to produce artificial spider silk has long been a dream of many researchers, but all attempts have until now involved harsh chemicals and have had limited results. Now, a team of researchers from the Swedish University of Agricultural Sciences and Karolinska Institutet claims to have developed a method that works. They report that they can produce kilometer long threads that resemble real spider silk.

The results were recently published in the journal Nature Chemical Biology.

Spider silk is an attractive material–it is well tolerated when implanted in tissues, it is light-weight but stronger than steel, and it is also biodegradable. Spiders though, are difficult to keep in captivity and they spin small amounts of silk.

Any large scale production must involve the use of artificial silk proteins and spinning processes. A biomimetic spinning process is probably the best way to manufacture fibers that resemble real spider silk. Until now, this has not been possible because of difficulties to obtain water soluble spider silk proteins from bacteria and other production systems, and therefore strong solvents has been used in previously described spinning processes.

Spider silk is made of proteins that are stored as an aqueous solution in the silk glands, before being spun into a fiber. Researcher Anna Rising and her colleagues Jan Johansson and Marlene Andersson at the Swedish University of Agricultural Sciences and at Karolinska Institutet have previously shown that there is an impressive pH gradient in the spider silk gland, and that this well-regulated pH gradient affects specific parts of the spider silk proteins and ensures that the fiber forms rapidly in a defined place of the silk production apparatus.

This knowledge has now been used to design an artificial spider silk protein that can be produced in large quantities in bacteria. This, in turn, enables the production of the material in an industrial quantity.

"This artificial protein is as water soluble as the natural spider silk proteins, which means that it is possible to keep the proteins soluble at extreme concentrations."

"To our surprise, this artificial protein is as water soluble as the natural spider silk proteins, which means that it is possible to keep the proteins soluble at extreme concentrations", says Anna Rising.

To mimic the spider silk gland, the research team constructed a simple but very efficient and biomimetic spinning apparatus in which they can spin kilometer-long fibers only by lowering the pH.

"This is the first successful example of biomimetic spider silk spinning. We have designed a process that recapitulates many of the complex molecular mechanisms of native silk spinning. In the future this may allow industrial production of artificial spider silk for biomaterial applications or for the manufacture of advanced textiles", says Rising.